10x vortex adapter  (10X Genomics)

Journal: Nature protocols

Article Title: Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

doi: 10.1038/s41596-022-00795-3

Figure Lengend Snippet: To retain mtDNA within their host cells, whole cells are fixed before mild lysis and permeabilization. Permeabilization enables access of the Tn5 transposase for transposition of nuclear accessible chromatin and mtDNA. Tagmented cells are encapsulated into droplets by using the Chromium Next GEM Single Cell ATAC platform by 10x Genomics to achieve single-cell compartmentalization and library generation.

Article Snippet: 10x vortex adapter (10x Genomics, cat. no. 120251 or 330002).

Techniques: Lysis

Journal: Nature protocols

Article Title: Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

doi: 10.1038/s41596-022-00795-3

Figure Lengend Snippet: Troubleshooting table

Article Snippet: 10x vortex adapter (10x Genomics, cat. no. 120251 or 330002).

Techniques: Gentle, Cell Recovery, Lysis, Incubation, High Molecular Weight, Sequencing, Gradient Centrifugation, Selection, DNA Purification

Journal: Nature protocols

Article Title: Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

doi: 10.1038/s41596-022-00795-3

Figure Lengend Snippet: a, Typical fragment size distribution of a conventional 10x Genomics scATAC-seq library with prominent peaks indicative of the nucleosomal banding of chromatin. b, Typical fragment size distribution of mtscATAC-seq libraries, with the first peak (nucleosome-free DNA) often, but not always, being the most prominent, given the additional enrichment of mitochondrial DNA. Libraries were run on a high-sensitivity DNA chip and the Agilent Bioanalyzer 2100 system. FU, fluorescence units.

Article Snippet: 10x vortex adapter (10x Genomics, cat. no. 120251 or 330002).

Techniques: Fluorescence